This lesson discusses what DNA is and how it relates to genes and chromosomes. How and why DNA is extracted in the genetic engineering process is also covered.
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This lesson contains information about the history, life cycle, and host plants of the European corn borer and information relating to the history and biology of Bacillus thuringiensis.
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This lesson covers the utilization of gene cloning to isolate and copy a specific gene of interest. The transformation of bacteria with plasmids containing antibiotic resistance genes to make gene libraries and the selection of bacteria colonies that contain the specific gene of interest are described.
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This lesson describes the three gene regions and their roles in gene expression. It also discusses how the regions of a gene can be altered to obtain desired trait expression.
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This lesson builds upon the gene region lesson discussing the gene construct of currently used hybrids and explaining how these combinations result in a particular gene expression.
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Lesson one is a general description of the overall process of genetic engineering. A basic explanation of the five steps for genetically engineering a crop is provided. Details for each step are given in later lessons of this course. The five steps are:
- Locating an organism with a specific trait and extracting its DNA
- Cloning a gene that controls the trait
- Designing a gene to express in a specific way
- Transformation, inserting the gene into the cells of a crop plant
- Plant breeding to get the transgene into an elite background
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This lesson explains the technique of tissue culture as used in plant transformation. It discusses important issues, such as the use of selectable markers, genotype specificity, and tissue culture alternatives.
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